The role of helper lipids in cationic liposomemediated. Liposomemediated cftr gene transfer to the nasal epithelium of patients with cystic fibrosis skip to main content thank you for visiting. Increased geneexpression liposomemediated arterial. Ultrasound mediated gene and drug delivery using a microbubble liposome particle system. National cancer center resenrch institute, tstikiji 5ihtme. Repeated cationic multilamellar liposome mediated gene transfer enhanced transduction efficiency against murine melanoma cell lines journal of dermatological science, vol. Liposome mediated gene transfer in rat lung transplantation.
Jun 26, 2018 liposome mediated gene delivery is the way of transfering gene into the liposome to mediate gene into the cell and perform its desired function. Many liposome based dna delivery systems have been described, includ ing molecular. Liposomemediated gene transfer and expression via the skin, human molecular genetics, volume 4, issue 12. Possible mechanism of polycation liposome pclmediated gene. Agrobacterium mediated gene transfer in plants biotechnology. It can also include the use of polymeric gene carriers polyplexes. Progress has been made toward developing nonviral, pharmaceutical formulations of genes for in vivohuman therapy, particularly cationic liposome mediated gene transfer systems. Characterization of cationic liposomemediated gene. Supercoiled wide range of applications for gene therapy. Physical gene transfer methodselectro portion, particle bombardment, microinjection, liposome fusion, silicon carbide fibres. Dechorionated zygotes and early fish embryos were treated with the transforming.
This has encouraged the development of nonviral dnamediated gene transfer techniques such as liposomes. Liposomes are artificial spherical vesicles which contain a small volume of aqueous solution enclosed in a lipid bilayer. A lipofection approach for gene transfer into primary endothelial cells. Liposomemediated gene transfer was used to introduce large dna constructs into zygotes of african catfish.
Liposome mediated transfer into the nucleusis still not completely understood. Cholesterol is an important cell membrane component and has been used as colipid for cationic liposome to enhance gene delivery. Despite the growing body of research on the biophysical and biochemical factors involved in cationic liposome mediated gene transfer, such as structural features, cellular association, and intracellular trafficking, the relationships between these factors and the mechanisms of lipoplex internalization into cells are not yet fully understood. A human hgf expression plasmid vector was encapsulated in hemagglutinating virus of japan hvj liposome, and injected into skeletal muscles of mice to obtain persistent high plasma level of hgf in mice.
The dna liposome mediated gene transfer has already been used for the delivery of recombinant genes into cells and tissues and shown to have a number of notable advantages. As compared to other methods of gene transfer used in the late 1980s. We show here a therapeutic effects of hgf on ibd animal model in mice by hvj liposome mediated hgf gene transfer into skeletal muscles. The use of a cation is a recurring theme for virtually all chemically mediated gene delivery vectors, including polymers, lipids, and nondegradable nanoparticles. Highly efficient liposomemediated gene transfer of. Liposomemediated gene transfer and expression via the skin. Agrobacterium is a gramnegative pathogenic bacteria involved in causing crown gall formation disease in plant species. Such fusogenic particles have been termed virosomes. Liposome mediated gene transfer liposome are small lipid bags, in which large number of plasmids are prepared artificially. Isolated lung liposomemediated gene transfer produces organ. Young il yoon 1,2,3, yongsu kwon 4, heesang cho 4, sunhee heo 5, kyeong soon park 5, sang gyu park 5, soohong lee 5, seung il hwang 1,2, young il kim 1, hwan jun jae 1, gookjun ahn 7, youngseok cho 8, hakho lee 9, hak jong lee 1,2,3,10, tae. We have recently studied the feasibility, safety, and pattern of liposome mediated gene transfer in a rat lung transplant model. Many liposome based dna delivery systems have been described, includ.
Between0 and 14 d after balloon angioplasty, percutaneousarterial genetransferwasperformed. Gene transfer technique mybiosource learning center. Nonviral methods include physical methods such as electroporation, microinjection, gene gun, impalefection, hydrostatic pressure, continuous infusion, and sonication and chemical, such as lipofection, which is a lipid mediated dnatransfection process utilizing liposome vectors. Many liposome based dna delivery systems have been described, including molecular components for targeting given cell surface receptors or for escaping from the lysosomal compartment. This is different from the vertical gene transfer where the genetic material is transferred from the parents to the offsprings, through sexual reproduction. On treatment of dna fragment with liposomes, the dna pieces get encapsulated inside liposomes. However, the role of cholesterol in transfection efficiency has not been fully understood.
A comparison between the in vivo and ex vivo approaches. The foreign dna is either randomly inserted into the host genome or recombines if there is sequence homology between the two genomes. Liposome mediated gene transfer into the rat oesophagus gut. In vivo and ex vivo liposome mediated gene transfer to lung isografts allows significant transgene expression with minimal effects on graft function. Pdf liposomes have a wide array of uses that have been continuously expanded and. Transformation is the genetic alteration of a cell resulting from the introduction, uptake and expression of foreign genetic material dna or rna.
The success of liposomemediated gene delivery is a multifactorial issue and. Liposome mediated gene transfer liposomes are spheres of lipids which can be used to transport molecules into the cells. Therefore, the present study was designed to improve cationic liposome mediated transfection of rabbit vascular. They appear most effective at delivering dna to cells when manufactured as a combination of cationic positively charged and neutral lipids. This provides a useful model to study the effects of gene. Introduction pancreatic cancer has continued to be one of the most difficult cancers today with a grave patient prognosis of gene was transduced in place of the hsvtk gene, the bluestained cells were identified only in tumor nodules and not in normal organs. Drake dept, of animal and poultry science, university of guelph, guelph, ontario, canada, nig 2w1 summary we have previously reported on factors affecting the efficiency of liposome mediated gene transfer to chicken sperm cells. Liposomemediated gene transfer to lung isografts core. Furthermore, the improvement of renal function in uuo demonstrates that the transfected inos gene is active and suggests that decreased inos activity contributes to the decreased renal function in uuo.
The main conclusion from the present results is that liposome based gene delivery strategy could be used together with aav mediated gene. The efficiency of liposome mediated gene transfer can be enhanced by incorporating viral proteins that facilitate the active fusion between viral envelopes and cell membranes. The role of helper lipids in cationic liposomemediated gene. Rabbitswereanesthetized with ketamineandacepromazine. Characterization of liposomemediated gene delivery nature. These are artificial vesicles that can act as delivery agents for exogenous materials including transgenes. Attempts to perform arterial gene transfer using nonviral vectors have been compromised by a low transfection efficiency. The crown gall formation is due to the transfer of a segment of oncogenic cancer causing dna into the plant cell at wounded sites. Jci increased gene expression after liposomemediated. Promote transport after fusing with the cell membrane. This has encouraged the development of nonviral dna mediated gene transfer techniques such as liposomes. The incision was then closed after the kidney was iod of gene expression as shown.
The same plasmids prsvluc,pgsvlaczused for arterial genetransfer in organculture were used for in vivo arterial gene transfer. Cationic liposome mediated gene transfer may become a favorable transfection technique with regard to patients safety for in vivo administration. Liposomes are often used to deliver a molecular cargo such as dna for therapeutic benefit. Liposomemediated transfer of nitric oxide synthase gene. Cationic liposomemediated gene transfer may become a favorable transfection technique with regard to patients safety for in vivo administration. Slim microscopy allows for visualization of dnacontaining. Vector mediated gene transfer is carried out either by agrobacterium mediated transformation or by use of plant viruses as vectors. Basis of pulmonary toxicity associated with cationic lipid mediated gene transfer to the mammalian lungbasis of pulmonary toxicity associated with cationic lipid mediated gene transfer to the mammalian lung. The role of helper lipids in cationic liposomemediated gene transfer.
These arrangements can be found in many shapes and sizes depending on lipid composition. The salient features of the commonly used gene dna transfer methods are given in table 49. Pcls show notable transfection efficiency with low cytotoxicity. Ultrasound mediated gene and drug delivery using a microbubble liposome particle system young il yoon 1,2,3, yongsu kwon 4, heesang cho 4, sunhee heo 5, kyeong soon park 5, sang gyu park 5, soohong lee 5, seung il hwang 1,2, young il kim 1, hwan jun jae 1, gookjun ahn 7, youngseok cho 8, hakho lee 9, hak jong lee 1,2,3,10.
Nov 15, 20 the transferred gene is known as transgene and the organism that develop after a successful gene transfer is known as transgenic. A 3galactosidase gene expression construct was used to investigate the effectiveness of gene delivery and expression when dnaliposome complexes were. Liposome mediated gene transfer was used to introduce large dna constructs into zygotes of african catfish. Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively. Liposome mediated in vivo gene transfer of antisense kras construct inhibits pancreatic tumor dissemination in the murine peritoneal cavity1 kazunori aoki, teruhiko yoshida, takashi sugimura, and masaaki terada2 genetics division. Possible mechanism of polycation liposome pclmediated gene transfer. Transferrinliposomemediated systemic p53 gene therapy. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Liposome lipidbased formulation has the least influence. Methods of gene transfer lesson prepared under mhrd. Liposomemediated in vivo gene transfer of antisense kras. Liposomes may offer several advantages as vectors for gene delivery into plant cells.
The liposome can be used as a vehicle for administration of nutrients and pharmaceutical drugs. The technique is based on the delivery of recombinant bacteriophage lambda particles or. Abstract a highefficiency, nonviral gene transfer protocol employing cationic liposome plus a receptor ligand is described. Furthermore, the present data also illustrated that the inhibition effect of ta was most likely due to the interference of early stages during viral infection. Isolated lung liposomemediated gene transfer produces. Liposome mediated gene transfer has been shown to be a highly efficient method for transforming cultured cells with foreign dna. Highly efficient liposomemediated gene transfer of inducible. The main conclusion from the present results is that liposome based gene delivery strategy could be used together with aav mediated gene therapy.
Characterization of liposomemediated gene delivery. Praveen sapkota iaas, tu rampur, chitwan, nepal a plant in which a gene has been transferred through genetic engineering is called a transgenic plant. Liposomemediated gene transfer and expression via the skin m. Basis of pulmonary toxicity associated with cationic lipidmediated gene transfer to the mammalian lungbasis of pulmonary toxicity associated with cationic lipidmediated gene transfer to the mammalian lung. However, the mechanism of pcl mediated gene transfer is still unclear. Polycation liposome mediated gene transfer in vivo. Hepatocyte growth factor hgf is known to have multifunctional effects such as mitogenic, antiapoptotic, and antiinflammatory activities. Liposomes consist mainly of phospholipids and cholesterol, major components of biological membranes.
We also showed that gene therapy techniques can bring. Chemical gene transfer methodspolyethylene glycol peg mediated, diethyl amino ethyl deae dextranmediated, calcium phosphate precipitation. A placebocontrolled study of liposome mediated gene transfer to the nasal epithelium of patients with cystic fibrosis. Liposomes have a wide array of uses that have been continuously expanded and improved upon since first being observed to selfassemble into vesicular structures. We have characterized a new synthetic gene delivery system, termed dls, which may be suitable for systemic gene therapy. Pdf liposomes as a gene delivery system researchgate. Pdf polycation liposomemediated gene transfer in vivo. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle.
Get a printable copy pdf file of the complete article 2. The major advantage to sperm mediated transfer for producing transgenic animals is its simplicity. Efficient delivery of nucleic acids to cells in a culture dish. To develop gene therapy targeted to the kidney, we compared three different routes of liposome mediated gene delivery to the kidney in mice, ie intrarenalpelvic, intrarenalarterial, and intra. Liposome lipidbased formulation has the least influence on. Dnamediated gene transfer techniques such as liposomes. The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer. Liposomes have also been a very effective tool to transfect spermatozoa 19. The use of a cation is a recurring theme for virtually all chemically mediated gene delivery vectors. Pdf kidneytargeted liposomemediated gene transfer in mice. This preclinical study suggests the therapeutic feasibility of the i.
Medical and biological safety concerns are thereby minimised. These liposomes can adher to cell membranes and fuse with them to transfer dna fragments. Liposomemediated cftr gene transfer to the nasal epithelium. This provides a useful model to study the effects of gene therapy on pulmonary tumors, which may have further application when gene therapy is used in clinical practice. A liposome is a spherical vesicle having at least one lipid bilayer. Furthermore, integration of antigenic proteins into liposome membranes is being used for vaccination against virus infection. S w hui, m langner, y l zhao, p ross, e hurley, and k chan. Hemagglutinating virus of japan liposomemediated gene. Enhancement of liposome mediated gene transfer by adding. Liposomemediated gene transfer in rat lung transplantation. Liposome mediated transfection was the first nonviral technique devised specifically for in vivo dna transfer. Now, genetic advances are heralding a new application to the use of liposomes, namely liposomemediated gene delivery.
The technique is based on the delivery of recombinant bacteriophage lambda particles or dnaprotamine complexes into the cytoplasm of target cells by negatively charged, large unilamellar liposomes. Laboratory for gene therapy and cellular engineering, department of chemical and. Possible mechanism of polycation liposome pclmediated. A placebocontrolled study of liposomemediated gene transfer. Methods of gene transfer dna transfer by natural methods 1.
Read at the seventyeighth annual meeting of the american association for thoracic surgery, boston, mass, may 36, 1998. We investigated the hypothesis that cellular proliferation induced by arterial injury could augment gene expression after liposome mediated gene transfer. The liposome mediated gene transfer, referred to as lipofection, is depicted in fig. Possible mechanism of polycation liposome pcl mediated gene transfer. Characterization of liposome mediated gene delivery. By continuing to use our website, you are agreeing to our use of cookies. This study shows that the liposome mediated in vivo gene transfer of antisense kras construct may be a useful therapeutic strategy for pancreatic cancer. Liposomes can be prepared by disrupting biological membranes such as by sonication. A novel gene transfer system utilizing polycation liposomes pcls, obtained by modifying liposomes with cetyl polyethylenimine pei, was previously developed gene ther. Liposomemediated gene transfer in fish embryos springerlink. They can be induced to fuse with protoplast using devices like peg, therefore have been used for gene transfer.
Effects of retinyl acetate on surface morphology and intramembrane particle distribution in the plasma membrane of 10t12 cells. Arterial oxygen tension was normal in treated animals. Pdf liposomes for use in gene delivery researchgate. Enhancement of liposome mediated gene transfer by adding cholesterol and cholesterol modulating drugs author links open overlay panel yunui bae a 1 jaewan huh b 1 bieongkil kim c hyeon young park a youngbae seu c kyungoh doh a. Inflammation was minimal, and tumor necrosis factor. Liposome mediated cftr gene transfer to the nasal epithelium of patients with cystic fibrosis natasha j.